추천 제품
양식
ammonium sulfate suspension
Quality Level
품질
crude extract
분자량
94 kDa
농도
5-30 units/mL
외래 활성
β-N-acetylglucosaminidase and α-mannosidase <1.0%
저장 온도
2-8°C
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일반 설명
β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.
애플리케이션
β-Mannosidase from Helix pomatia has been used:
- in the digestion of glycans for enzymatic analysis
- in the treatment of labeled monogalactosyl Manno-oligosaccharides
- in non-mammalian glycosidase inhibition assays
생화학적/생리학적 작용
β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.
단위 정의
One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.
물리적 형태
Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
Umme Afsari Zahura et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 162(1-3), 24-33 (2012-03-01)
A β-D-mannosidase (EC 3.2.1.25) with a molecular mass of approximately 100 kDa was purified from the digestive fluid of a marine gastropod Aplysia kurodai by ammonium sulfate fractionation followed by column chromatographies on TOYOPEARL Butyl-650 M, TOYOPEARL DEAE-650 M, and
Sunghoon Park et al.
Protein and peptide letters, 20(1), 107-114 (2012-07-14)
Sequence alignments and homology modeling of Pyrococcus furiosus thermostable glycosidase (PFTG) showed that the residue 150 is conserved as tryptophan in β-glycosidase and in other related enzymes such as β- mannosidase and β-galactosidase. To elucidate the relationship between the substrate
Barbora Fliedrová et al.
Protein expression and purification, 85(2), 159-164 (2012-08-14)
β-Mannosidase (EC 3.2.1.25) is an exoglycosidase specific for the hydrolysis of terminal β-linked mannoside in various sugar chains. cDNA corresponding to the β-mannosidase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Pichia pastoris. The β-mannosidase gene
Farage H Al-Ghazzewi et al.
Journal of the science of food and agriculture, 92(11), 2394-2396 (2012-04-13)
Glucomannan polysaccharides may be hydrolysed to lower molecular weight molecules using acids or enzymes, specifically mannanases or cellulases. Mannanases (β-mannanases) hydrolyse β-(1-4)-linked mannose residues randomly in mannans whilst cellulases (β-glucanase) hydrolyse β-(1-4)-linked glucose residues. The molecular weight of the hydrolysate
Cristina Martínez-Andújar et al.
The Plant journal : for cell and molecular biology, 71(4), 575-586 (2012-03-31)
The micropylar region of endosperm in a seed, which is adjacent to the radicle tip, is called the 'endosperm cap', and is specifically activated before radicle emergence. This activation of the endosperm cap is a widespread phenomenon among species and
문서
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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