G6920

Endo-β-galactosidase from Bacteroides fragilis

recombinant, expressed in E. coli, ≥140 units/mg protein, buffered aqueous solution

• 동의어(들): β-Galactosidase bacterial, Keratanase
• EC 번호: 3.2.1.103 (BRENDA, IUBMB)
• MDL number: MFCD00131433
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 결합: (Glucosaminoglycan)
• 무균: aseptically filled
• 양식: buffered aqueous solution
• 특이 활성도: ≥140 units/mg protein
• 분자량: 32 kDa
• 저장 온도: 2-8°C

설명

애플리케이션

Endo-β-galactosidase was used in fractional protein isolation. It was used for deglycosylation in glycoproteomics of the endothelial secretome of human endothelial cells.

생화학적/생리학적 작용

Internal β(1-4) galactose linkages in unbranched, repeating poly-Nacetyllactosamine [GlcNAc β(1-3)Gal β (1-4)] structures are the preferred substrate.

Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may reduce or completely inhibit cleavage. Sulfation of C-6 on galactose will block cleavage. Oligosaccharides of the neolacto-group are cleaved at greatly reduced rates depending on the deviation from the preferred substrate. For example, Gal β(1-3)GlcNAc β(1-3) Gal β(1-4)Glc is cleaved at 5X10^-5 the rate of keratan sulfate

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

단위 정의

One unit will release 1.0 μmole of reducing sugar from bovine corneal keratan sulfate per minute at 37 °C, pH 5.8.

물리적 형태

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)