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Merck
모든 사진(4)

주요 문서

F2168

Sigma-Aldrich

Anti-α-Tubulin−FITC antibody, Mouse monoclonal

clone DM1A, purified from hybridoma cell culture

동의어(들):

Monoclonal Anti-α-Tubulin

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

FITC conjugate

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

DM1A, monoclonal

양식

buffered aqueous solution

분자량

antigen ~50 kDa

종 반응성

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

저장 조건

protect from light

기술

direct immunofluorescence: 1:50 using cultured BHK cells

동형

IgG1

UniProt 수납 번호

응용 분야

research pathology

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

α/β-Tubulin, the major building block of microtubules, belongs to the tubulin superfamily of proteins. They are ubiquitously found in all eukaryotic cells. It exists in multiple isotype in many organisms.† It is a heterodimer with intracellular, hollow, cylindrical, filamentous structures. It is located on human chromosome 2q35. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β--Tubulin is a heterodimer, which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog. Tubulin is a highly conserved protein with two terminal domains: large N and C terminal.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC.

특이성

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.
The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.

면역원

Microtubules from chicken embryo brain.
microtubules from chicken embryo brain.

애플리케이션

Monoclonal Anti-α-Tubulin antibody is suitable for immunostaining of:
  • respiratory epithelium tissue in a study to determine the tubulin expression in the mice cilia
  • breast cancer tissue sections to study the effect of LMO4 on the centrosome amplification and mitotic spindle abnormalities
  • spindle and chromosomes of oocytes
Mouse monoclonal antibody has been used in the:
  • detection of tubulin by immunofluorescence and confocal microscopy in lung carcinoma cell.
  • immunofluorescent staining of microtubules in human embryos and mitotic spindles from spleen lymphoblast.

생화학적/생리학적 작용

The N-terminal domain of α−tubulin binds GTP, which is non-exchangeable mechanism, whereas highly acidic C-terminal domain is capable to bind microtubule-associated proteins (MAPs). Both domains are associated with the microtubular self-assembly. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. Mutations in tubulin α-4A gene leads to a neurodegenerative disease called amyotrophic lateral sclerosis.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide

저장 및 안정성

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing , or storage in "frost-free" freezers. is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

H Masuda et al.
The Journal of cell biology, 110(2), 417-425 (1990-02-01)
To investigate the mechanisms of spindle elongation and chromosome separation in the fission yeast Schizosaccharomyces pombe, we have developed an in vitro assay using a temperature-sensitive mutant strain, nuc2. At the restrictive temperature, nuc2 cells are arrested at a metaphase-like
Downregulation of survivin and aurora A by histone deacetylase and RAS inhibitors: a new drug combination for cancer therapy.
Biran A, et al.
International Journal of Cancer. Journal International Du Cancer, 128(3), 691-701 (2011)
A revised nomenclature for the human and rodent a-tubulin gene family.
Khodiyar V K, et al.
Genomics, 90(2), 285-289 (2007)
Y F Inclán et al.
Journal of cell science, 114(Pt 2), 413-422 (2001-01-10)
alphabeta-tubulin heterodimers self-assemble to form microtubules nucleated by gamma-tubulin in the cell. Gamma-tubulin is believed to recruit the alphabeta-tubulin dimers that form the minus ends of microtubules, but the molecular mechanism of this action remains a matter of heated controversy.
Lingyan Wang et al.
Bioscience reports, 29(5), 315-320 (2008-11-05)
The golden hamster is an attractive model organism for studying reproductive physiology, oncology, genetics and virology. In an effort to establish experimental protocols necessary for cloning golden hamsters, we examined changes in the reciprocal position of the FPB (first polar

문서

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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