Name: DAPI
Brand: SIGMA

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About This Item

실험식(Hill 표기법):

C₁₆H₁₅N₅ · 2HCl

CAS Number:

28718-90-3

Molecular Weight:

350.25

Beilstein:

4894417

EC Number:

249-186-7

MDL number:

MFCD00012681

UNSPSC 코드:

12352200

PubChem Substance ID:

24894305

NACRES:

NA.52

기술 서비스

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도움 문의

Grade

Molecular Biology

Quality Level

300

분석

≥98% (HPLC)

양식

powder

기술

transfection: suitable

solubility

H₂O: 20 mg/mL
PBS: insoluble

ε (흡광계수)

30 at 263 nm in H₂O at 1 mM

형광

λ_ex 340 nm; λ_em 488 nm (nur DAPI)
λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)

적합성

suitable for fluorescence

저장 온도

2-8°C

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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일반 설명

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.

애플리케이션

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Suitable for

DNA staining in agarose gels
analysis of changes in DNA during apoptosis
detection of mycoplasma
photofootprinting of DNA
immunofluorescent staining of cells

DAPI has been used:-

in rapid monitoring of microbial contamination
in chromosomal banding technique
in detection of apoptotic cells
in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
to stain mature pollen grains(0.5 mg/ml)

생화학적/생리학적 작용

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.