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Merck

RIP

Sigma-Aldrich

Imprint® RNA Immunoprecipitation Kit

High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA

Synonyma:

Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.54

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Quality Level

200

General description

Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.

Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.

Application

Imprint® RNA Immunoprecipitation Kit has been used for:
  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

Features and Benefits

  • Xtra Protein A magnetic beads with higher binding capacity
  • Step-by-step protocols for cell lysis and immunoprecipitation
  • RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
  • Negative control and antibody included

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list
  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderBezpečností list
  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderBezpečností list
  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionBezpečností list
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionBezpečností list
  • R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μLBezpečností list

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GHS02,GHS07

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Warning

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

Skladovací třída

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

100.4 °F

flash_point_c

38 °C

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