PF0100

Protease Fluorescent Detection Kit

high sensitivity assay

• Synonyma: Protease Assay Kit
• UNSPSC Code: 12352202
• NACRES: NA.26

Vlastnosti

• Quality Level: 200
• shipped in: dry ice
• storage temp.: −20°C

Popis

Application

Fluorescent Protease Detection Kit Video Protocol

Protease Fluorescent Detection Kit has been used:

• in the detection of proteolytic activity using casein based protease assay
• for testing the extracellular proteolytic activity of fungal strains
• for quantifying mitochondrial protease activity
• for measuring the protease production from soil solution

Suitability

Detection limit: approx. 50 ng trypsin

Quantity

Supplied with sufficient reagents for up to 200 one mL assays.

Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Analysis Note

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Pouze součásti soupravy

• Incubation buffer 25 mL
• FITC-casein solution 5 mL
• Assay buffer 200 mL
• Protease standard (trypsin) 20 μg
• FITC control 50 mg
• Trichloroacetic acid (TCA) solution 30 mL

Bezpečnostní informace

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H314,H317,H334,H335,H410
• pcodes: P261 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Skladovací třída: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable