1.10316

Fractogel^® EMD TMAE Hicap (M)

• Synonyma: Biochromatography, Fractogel^® Ion exchangers, Ion exchanger for LC (Fractogel^®TSK/EMD), Fractogel^® EMD TMAE Hicap (M)
• UNSPSC Code: 41115711

Vlastnosti

• ligand: (Trimethylammoniumethyl)
• Quality Level: 400
• description: strong anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
• sterility: sterile (Caustic Stable)
• form: resin
• specific analyte(s): MAbs
• parameter: 180 cm/hr flow rate; 8 bar max. pressure
• matrix active group: methacrylate
• mean particle size: 40-90 μm
• capacity: 180 mg binding capacity (BSA/mg of resin)
• transition temp: flash point 35 °C (calculated)
• density: 1.430 g/cm³ at 20 °C
• bulk density: 1000 kg/m³
• application(s): recombinant protein
• separation technique: anion exchange; strong anion exchange
• storage temp.: 2-30°C

Popis

General description

Strong anion exchange Fractogel^® resin featuring the tentacle technology with a trimethylammoniumethyl functional group for purification of acidic and neutral proteins and peptides from multiple sources, DNA and virus removal, plasmid purification, vaccine purification, and more

Features and Benefits

Fractogel^® EMD TMAE Hicap (M) enables users to:

• Efficiently capture target protein and remove viruses, DNA, and endotoxins with its tentacle technology resulting in better production yields and high throughput
• Reduce operating costs and resin replacement with enduring durability - allowing for multiple cycles of column regeneration and sanitization
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior of Fractogel^® EMD TMAE Hicap (M) the ion exchange capacity can be used from pH 2 to 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD TMAE Hicap (M) when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Packaging

• 1.10316.0100: Fractogel^® EMD TMAE Hicap (M) Resin 100ml
• 1.10316.0010: Fractogel^® EMD TMAE Hicap (M) Resin 10ml
• 1.10316.0500: Fractogel^® EMD TMAE Hicap (M) Resin 500ml
• 1.10316.5000: Fractogel^® EMD TMAE Hicap (M) Resin 5L

Plastic bottle

Analysis Note

Appearance: Milky turbid suspension, free from impurities (foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.03 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (bovine serum albumin): 160 - 200 mg/ml
Functional test (b:a): ≤ 0.25
Functional test: Separation of conalbumin and human serum albumin

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Skladovací třída: 3 - Flammable liquids
• wgk_germany: WGK 1
• flash_point_f: 95.0 °F
• flash_point_c: 35 °C