SAE0219

Mucin Degradation Enzyme

for preparative digestions, powder

• Code UNSPSC : 41121800

Propriétés

• Niveau de qualité: 200
• Forme: powder
• Durée de conservation: 2 yr (retest)
• Conditions de stockage: protect from light
• Conditions d'expédition: dry ice

Description

Description générale

Mucin Degradation Enzyme is a metalloprotease useful for digesting heavily O-glycosylated mucin proteins. This product is made for preparative scale digestions of viscous material that is dense with mucin-type glycoproteins.

Application

For use as a sample preparation tool in research and discovery. Used for proteolytic degradation of mucin-dense samples to allow for downstream processing.

Actions biochimiques/physiologiques

The Mucin Degradation is based on a bacterial protease that specifically recognizes densely glycosylated mucins and mucin-domains, and proteolytically cleaves the peptide backbone. In nature this presumably enhances the virulence of the pathogenic microbe, but in the laboratory is a useful way of accessing molecular structures in tissues normally shielded by mucins.

Caractéristiques et avantages

• A promiscuous Mucin specific protease.
• Tool for viscosity reduction and tissue breakdown of mucin containing samples.
• Not inhibited by most general protease inhibitors such as AEBSF, Aprotinin, Bestatin, E-64, Pepstatin and Leupeptine. When needed digests can be done in presence of the ReadyShield^® Protease Inhibitor Cocktail PIC0002.
• Mucin Degradation Enzyme can be used to liberate soluble mucin fragments from cells or tissue without mechanical disruption or reduction.
• Mucin Degradation Enzyme can be used for enzymatic hydrolysis of mucins in the isolation of glyco-RNA samples.

Forme physique

Product is supplied as lyophilized powder containing the indicated amount of protein.

Notes préparatoires

Solution of Mucin Degradation Enzyme can be prepared by reconstitution of the lyophilized powder at a concentration of 1-5mg/ml protein. See CoA for lot specific percent protein in powder. The reconstituted protein solution may be clear or hazy and is stable for 2 weeks at 2-8 °C. For longer storage, aliquot the protein solution and store at -20 °C. The enzyme solution should be protected from direct light.

Stockage et stabilité

The product retains activity for at least 2 years when stored lyophilized at -20°C.

Autres remarques

For applications that require more precision with highly purified enzyme like glycoproteomic experiments please consider using Mucinase StcE (SAE0202) and Mucinase SmE (SAE0220).

Informations légales

Under licensing from Stanford University.

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable