MABF2193

Anti-pan Dengue Virus (1-4) Antibody, Clone 323

clone 323

• Synonyme(s) : Dengue virus 1-4 NS1 protein, pan-DENV NS1 protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified antibody
• Clone: 323, monoclonal
• Poids mol.: ~48 kDa (calculated for NS1 protein; 378.71 kDa for the entire genome polyprotein.)
• Produit purifié par: using protein G
• Espèces réactives: human
• Isotype: IgG1κ
• Numéro d'accès UniProt: P17763/P29990/Q6YMS4/P09866

Description

Description générale

Dengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. Clone 323 displays reactivity to NS1 protein in all Dengue viruses (1-4).

Spécificité

Clone 323 is a mouse monoclonal antibody that detects all four serotypes of Dengue virus NS1 protein.

Immunogène

Purified recombinant Dengue virus NS1 protein expressed in mammalian cells.

Application

Flow Cytometry Analysis: A representative lot detected pan Dengue Virus (1-4) in Flow Cytometry applications

ELISA Analysis: A representative lot detected pan Dengue Virus (1-4) in ELISA applications

Qualité

Evaluated by ELISA in DENV1, DENV2, DENV3, and DENV4 NS1 recombinant protein.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.