361526-M

GSK-3β, Rabbit Skeletal Muscle, Recombinant, E. coli

GSK-3β, Rabbit Skeletal MuscleHis-Tag, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.

• Synonyme(s) : GSK 3β-Isozyme, Rabbit Skeletal Muscle, Recombinant, E. coli
• Code UNSPSC : 12352202

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Forme: liquid
• Activité spécifique: ≥250,000 units/mL
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Activité étrangère: DNase <10% (conversion of RFI DNA to RFII and linear forms); Phosphatases and Proteases, none detected; RNases <10% (RNA degradation)
• Température de stockage: −20°C

Description

Description générale

Note: 1 KU = 1000 units.

Recombinant, rabbit glycogen synthase kinase 3β expressed in E. coli. Glycogen synthase kinase 3β is a monomeric protein whose activity is independent of second messengers such as cAMP or IP₃. Recent studies demonstrate that, like the dual specificity MAP kinase, it autophosphorylates on Ser, Thr, and Tyr. It phosphorylates several exogenous substrates, including protein phosphatase 1, the regulatory domain of protein kinase A, myelin basic protein, protein phosphatase inhibitor 2, c-jun, c-myb, c-myc, CREB, CREM, p90^rsk, and microtubule-associated tau protein only on Ser and/or Thr, but not on Tyr residues. Plays a key role in Wnt and insulin signaling.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of GSK-3 required to catalyze the transfer of 1 pmol phosphate to CREB phosphopeptide KRREILSRRPS(P)YRP (200 µM), in 1 min at 30°C in a reaction volume of 25 µl.

Forme physique

In 50 mM NaCl, 30 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, 0.03% BRIJ^®-35 Detergent, pH 7.5

Reconstitution

Following initial thaw aliquot and freeze (-20°C).

Autres remarques

Dajani, R., et al. 2001. Cell105, 721.
Wang, Q.M., et al. 1995. Biochem. Biophys. Res. Commun.208, 796.
Fiol, C.J., et al. 1994. J. Biol. Chem.269, 32187.
Wang, Q.M., et al. 1994. J. Biol. Chem.269, 14566.
Wang, Q.M., et al. 1994. Biochemistry33, 143.
deGroot, R.P., et al. 1993. Oncogene8, 841.
Ishiguro, K., et al. 1993. FEBS Lett.325, 167.

Informations légales

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable