T3253
Trizma® hydrochloride
reagent grade, ≥99.0% (titration), crystalline
Synonym(s):
TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
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About This Item
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein/REAXYS Number:
3675235
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
grade
reagent grade
Quality Level
assay
≥99.0% (titration)
form
crystalline
storage condition
dry at room temperature
technique(s)
affinity chromatography: suitable
impurities
≤0.5% water (Karl Fischer)
color
white
useful pH range
7.0-9.0
pKa (25 °C)
8.1
mp
150-152 °C
solubility
H2O: soluble, clear, colorless
density
1.39 g/cm3 at 20—25 °C
1.41 g/cm3 at 20—25 °C
absorption
≤0.05 at 290 nm at 40%
suitability
suitable for mass spectrometry
application(s)
diagnostic assay manufacturing
life science and biopharma
sample preparation
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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General description
Tris HCl, or Trizma® hydrochloride, is a widely utilized acidic buffer in the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, it offers a pH range of 7.0 to 9.0, aligning with living organisms′ typical pH. Essential in various biological, cell biology, and biochemistry applications, Tris HCl is crucial for effective pH control in buffer solutions. Notably, Tris base and Tris hydrochloride alone lack sufficient buffering capacity, prompting their combination within the pH range of 7 to 9.
Tris buffers play a key role in techniques like protein electrophoresis and western blotting, serving in low ionic strength buffers for protein transfer. They are also integral to SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, especially in TAE and TBE buffers, are extensively employed in DNA agarose electrophoresis. These buffers are pivotal in diverse applications, including biochemistry, protein purification, and electrophoresis, showcasing the versatility and importance of Tris HCl in maintaining optimal pH for biochemical and cell biology research.
Tris buffers play a key role in techniques like protein electrophoresis and western blotting, serving in low ionic strength buffers for protein transfer. They are also integral to SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, especially in TAE and TBE buffers, are extensively employed in DNA agarose electrophoresis. These buffers are pivotal in diverse applications, including biochemistry, protein purification, and electrophoresis, showcasing the versatility and importance of Tris HCl in maintaining optimal pH for biochemical and cell biology research.
Application
Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.
Features and Benefits
- Ideal as a Biological buffer for Cell Biology and Biochemical research
- Effective Buffering from pH 7-9 with a pKa of 8.1 (25 °C)
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Packaging
The DRYPOUR®packaging system for high-quality hygroscopic salts DRYPOUR®packaging:
- The current DRYPOUR®packaging configuration is in a 50L nestable drum
- 25KG-DP are the standard prepack offering
- Smaller volumes can be put in the same drum for variable configurations
- Improves operational efficiency by reducing material preparation time and increasing operator safety
- Minimizes caking of hygroscopic salts
- Offers triple protection: against moisture inside and outside, and against contamination
- This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
- PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek®liner
- Desiccant bags are firmly integrated into the polyethylene liner
- Triple protection delivers two invaluable results:
- Dramatically reduced caking
- No contamination risk from the desiccant
Other Notes
For additional information on our range of Biochemicals, please complete this form.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company
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Storage Class
11 - Combustible Solids
wgk_germany
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flash_point_f
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flash_point_c
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Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix® Bis-Tris Gel Casting Kits
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