10812846001

Tris hydrochloride

>99% (titration), pH 7.0-9.0, suitable for FISH

• Synonym(s): Trizma^® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein/REAXYS Number: 3675235
• MDL number: MFCD00012590
• UNSPSC Code: 12161700
• PubChem Substance ID: 329749168

Properties

• assay: >99% (titration)
• form: solid
• packaging: pkg of 500 g
• manufacturer/tradename: Roche
• technique(s): FISH: suitable
• pH: 7.0-9.0
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• absorption: <0.02 at 300 nm at 100 mg/mL
• storage temp.: room temp
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

Application

Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer.

Features and Benefits

Crystals

Quality

Purity: >99% Tris-HCl (titrimetric)
Heavy metals: <1 ppm Pb and Fe

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable