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Key Documents

SRP0389

Sigma-Aldrich

PRMT6 active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym(s):

HRMT1L6, Histone-arginine N-methyltransferase 6, protein arginine N-methyltransferase 6

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

43 kDa

packaging

pkg of 20 μg

NCBI accession no.

NM_018137

UniProt accession no.

Q96LA8

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRMT6(55170)

General description

Protein arginine methyltransferase 6 (PRMT6) is a 41.9kDa protein with 375 amino acids. It is encoded by the gene mapped to human chromosome 1. It belongs to type I enzyme, which is a subclass of PRMT family. PRMT6 is localized exclusively in nucleus.
Human PRMT6 (GenBank Accession No. NM_018137), amino-acids 2-375 (end), with N-terminal His-tag, MW=43kDa, expressed in Escherichia coli.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Protein arginine methyltransferase 6 (PRMT6) catalyze the formation of asymmetric ω-NG,NG -dimethylarginine residues. In addition, PRMT6 show automethylation activity, which is unique among the members of PRMT family. PRMT6 mediates cigarette smoke extract (CSE)-triggered apoptosis and inflammation via di-methylation H3 arginine 2 (H3R2me2a) in human umbilical vein endothelial cells (HUVECs). PRMT6 plays a crucial role in various physiological processes such as transcriptional regulation, DNA repair and human immunodeficiency virus pathogenesis. Upregulated expression of the enzyme might be favorable for tumor growth and progression. Overexpression of PRMT6 has been observed in various type of malignancies including breast, cervix, bladder, prostate and lung cancer.

Physical form

Formulated in 200 mM imidazole, 20% glycerol, and 3 mM DTT.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
5104910308

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PRMT6 mediates CSE induced inflammation and apoptosis.
Kang N
International Immunopharmacology, 24(1), 95-101 (2015)
Identification and characterization of new molecular partners for the protein arginine methyltransferase 6 (PRMT6).
Lo Sardo A
PLoS ONE, 8(1), e53750-e53750 (2013)
The arginine methyltransferase PRMT6 regulates cell proliferation and senescence through transcriptional repression of tumor suppressor genes.
Stein C
Nucleic Acids Research, 40(19), 9522-9533 (2012)
The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity.
Frankel A
The Journal of Biological Chemistry, 277(5), 3537-3543 (2002)
Ernesto Guccione et al.
Nature, 449(7164), 933-937 (2007-09-28)
Eukaryotic genomes are organized into active (euchromatic) and inactive (heterochromatic) chromatin domains. Post-translational modifications of histones (or 'marks') are key in defining these functional states, particularly in promoter regions. Mutual regulatory interactions between these marks--and the enzymes that catalyse them--contribute

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