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관련 카테고리
일반 설명
Topoisomerase II α (TopoIIα) is a gene product with conserved catalytic activities and it promotes the progression of DNA damage. The α isoform is present in proliferating cells.
Topoisomerase II α is encoded by TOP2A gene, which is located on 17q21.2 in the human chromosome. It is essential for controlling the topological state of DNA by reversibly breaking the DNA strands during replication, recombination and transcription. TOP2A expression increases at late S phase and during G2/M of the cell cycle and decreases after mitosis. Polymorphism with TOP2A causes chromosome instability and tumorigenesis. TOP2A causes tumor proliferation and invasiveness in various cancer types like breast cancer, ovarian cancer and prostate cancer.
애플리케이션
Human topoisomerase II α has been used in DNA cleavage assay.
Topoisomerase II α has been used in a study to assess aging processes in the human brain. Topoisomerase II α has also been used in a study to investigate its activity in HIV-1 replication.
생화학적/생리학적 작용
Used to study DNA structure and topology.
단위 정의
One unit will decatenate 0.2 μg of kinetoplast DNA in 30 minutes at 37°C.
물리적 형태
Buffered aqueous glycerol solution, containing Trizma buffer (pH 7.7). Other components are present in the solution, and lot-specific information on these components may be obtained from Technical Service.
제조 메모
Recombinant enzyme overexpressed in a proprietary eukaryotic cell line.
법적 정보
TOPO is a registered trademark of Life Technologies
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Raj Kumar Mandraju et al.
Archives of biochemistry and biophysics, 470(1), 27-34 (2007-11-21)
Topoisomerase II alpha (TopoIIalpha) and Topoisomerase II beta (TopoIIbeta) isoforms are different gene products having conserved catalytic activities. The alpha isoform is present in proliferating cell, while beta isoform is predominantly present in non-proliferating cells namely neurons suggesting its role
The prognostic significance of topoisomerase II alpha protein in early stage luminal breast cancer.
An X, et al.
BMC Cancer, 18(1), 331-331 (2018)
Topoisomerase 2 alpha: a real predictor of anthracycline efficacy?
Romero A, et al.
Clinical And Translational Oncology, 14(3), 163-168 (2012)
Yoshihiko Ichikawa et al.
The Journal of clinical investigation, 124(2), 617-630 (2014-01-03)
Doxorubicin is an effective anticancer drug with known cardiotoxic side effects. It has been hypothesized that doxorubicin-dependent cardiotoxicity occurs through ROS production and possibly cellular iron accumulation. Here, we found that cardiotoxicity develops through the preferential accumulation of iron inside
Elisa Latorre et al.
The Journal of pharmacology and experimental therapeutics, 349(1), 99-106 (2014-02-05)
The protein kinase Cδ (PKCδ) interacts with and phosphorylates HuR, dictating its functionality. We show here that the genotoxic stimulus induced by doxorubicin triggers PKCδ interaction with HuR and leads to HuR phosphorylation on serines 221 and 318 and cytoplasmic
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