SRP0416

T4 Beta-glucosyltransferase

recombinant, expressed in E. coli, ≥83% (SDS-PAGE)

• 동의어(들): T4-phage beta-glucosyltransferase, UDP glucose-DNA ?-glucosyltransferase, hydroxymethyl cytosine
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 생물학적 소스: Escherichia coli
• 재조합: expressed in E. coli
• 분석: ≥83% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 41.6 kDa
• 포장: pkg of 100 μg
• NCBI 수납 번호: NP_049658
• UniProt 수납 번호: P04547
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: bacteriophage T4 ... B-GT(1258765)

설명

일반 설명

T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.

애플리케이션

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

생화학적/생리학적 작용

T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg²⁺, Mn²⁺ and Ca²⁺ activate this enzyme.

물리적 형태

Formulated in 200 mM imidazole and 20% glycerol.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H360D
• 예방조치 성명서: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable