S5816

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

• 동의어(들): SDF-1α/PBSF
• MDL number: MFCD02100631
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 제품명: Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse, SDF-1α, recombinant, expressed in E. coli, powder, suitable for cell culture
• 생물학적 소스: mouse
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: >97% (SDS-PAGE)
• 양식: powder
• 효능: 0.15-0.6 ng/mL ED₅₀
• 품질: endotoxin tested
• 분자량: predicted mol wt ~8 kDa
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: <1 EU/μgtested (LAL test)
• UniProt 수납 번호: P40224
• 저장 온도: −20°C
• 유전자 정보: mouse ... Cxcl12(20315)

설명

일반 설명

Stromal derived factor-1 (SDF-1) is localized to adipocytes.

애플리케이션

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse has been used in invasion assay. It has also been used to study the SDF-1 release from chitosan-based nanoparticles.

생화학적/생리학적 작용

SDF-1α/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins. SDF-1α and SDF-1β (together also known as SDF-1α/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α..

Stromal derived factor-1 (SDF-1) is an insulin-desensitizing factor, upregulated during both starvation and obesity. Exogenic SDF-1 reduces insulin-mediated signaling and glucose uptake. SDF-1 mediates migration of cortical interneurons and endothelial progenitor cells of the bone marrow through chemotaxis.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

제조 메모

Recombinant Mouse Stromal Cell-Derived Factor 1α (SDF-1α)/Pre-B Cell Growth Stimulating Factor (PBSF) is produced from a DNA sequence encoding the mature mouse SDF-1α protein sequence (amino acid residues 22-89) expressed in Escherichia coli. Recombinant mouse SDF-1α (68 amino acids) has a predicted molecular mass of approximately 8 kDa.

분석 메모

The biological activity is measured by its ability to chemoattract cultured human lymphocytes and mouse BaF3 cells transfected with human CXCR4.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)