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Merck
모든 사진(1)

주요 문서

R1780

Sigma-Aldrich

RPMI-1640 Medium

With ʟ-glutamine, sodium bicarbonate without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, suitable for isotope labeling for cell culture applications

동의어(들):

RPMI 1640 Cell Culture Medium, Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
UNSPSC 코드:
12352207
NACRES:
NA.75

제품명

RPMI-1640 Medium, With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

무균

sterile-filtered

양식

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

재구성

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Guokai Yan et al.
Scientific reports, 7(1), 1914-1914 (2017-05-17)
Leucine (Leu) is a multifunctional essential amino acid that plays crucial role in various cellular processes. However, the integral effect of Leu on the hepatic proteome remains largely unknown. Here, we for the first time applied an isobaric tags for
Rachel S Lee et al.
Neoplasia (New York, N.Y.), 21(4), 389-400 (2019-03-23)
Approximately 50% of prostate cancers harbor the TMPRSS2:ERG fusion, resulting in elevated expression of the ERG transcription factor. Despite the identification of this subclass of prostate cancers, no personalized therapeutic strategies have achieved clinical implementation. Kinases are attractive therapeutic targets
Zhang Xiao Ying et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 22(21), 5383-5393 (2016-11-03)
This study was performed to further our understanding of the biological and genetic basis of follicular lymphoma and to identify potential novel therapy targets. We analyzed previously generated whole exome sequencing data of 23 follicular lymphoma cases and one transformed
Hanlin Zhang et al.
Molecular cell, 76(1), 110-125 (2019-09-03)
Failure to make adaptive immune responses is a hallmark of aging. Reduced B cell function leads to poor vaccination efficacy and a high prevalence of infections in the elderly. Here we show that reduced autophagy is a central molecular mechanism
Michael D Lewis et al.
The American journal of tropical medicine and hygiene, 81(6), 1041-1049 (2009-12-10)
Trypanosoma cruzi, the agent of Chagas disease, can be subdivided into six discrete typing units (DTUs), TcI, TcIIa, TcIIb, TcIIc, TcIId or TcIIe, each having distinct epidemiologically important features. Dozens of genetic markers are available to determine the DTU to

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