애플리케이션
The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.
원리
The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.
수량
Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.
법적 정보
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
키트 구성품 전용
제품 번호
설명
- Acetonitrile, ≥99.93%, BioSyn® 50 mL
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
3 - Flammable liquids
Flash Point (°F)
35.6 °F
Flash Point (°C)
2.0 °C
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