P7367
PNGase F from Elizabethkingia meningoseptica
BioReagent, ≥95% (SDS-PAGE)
동의어(들):
N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
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모든 사진(1)
About This Item
CAS Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.32
추천 제품
생물학적 소스
bacterial (Elizabethkingia meningoseptica)
Quality Level
결합
(N-linked)
제품 라인
BioReagent
분석
≥95% (SDS-PAGE)
양식
powder
유통기한
≥1 weeks at 2‑8 °C (for a reconstituted solution >500 units/ml)
≥1 yr at 2‑8 °C
Solution is stable for at least 3 freeze-thaw cycles
분자량
~36 kDa
농도
≥300 units/mL
≥50 units/mL
최적 pH
~8.6
배송 상태
wet ice
저장 온도
2-8°C
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일반 설명
Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.
애플리케이션
PNGase F from Elizabethkingia meningoseptica has been used:
- for de-N-glycosylation of Zika20virus E protein
- to evaluate coxsackievirus and adenovirus receptor glycosylation using CAR-expressing COS cells
- to verify the N-linked glycosylation of MHC class 1 polypeptide-related sequence A (MICA)
Suitable for both proteomics and glycobiology use; compatible with MALDI-TOF MS analysis
Used to deglycosylate protein.
생화학적/생리학적 작용
Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
단위 정의
One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
Katherine J D Ashbourne Excoffon et al.
Journal of virology, 81(11), 5573-5578 (2007-03-23)
The coxsackievirus and adenovirus receptor (CAR) is both a viral receptor and homophilic adhesion protein. The extracellular portion of CAR consists of two immunoglobulin (Ig)-like domains, each with a consensus sequence for N-glycosylation. We used chemical, genetic, and biochemical studies
Lars Andresen et al.
Journal of immunology (Baltimore, Md. : 1950), 188(4), 1847-1855 (2012-01-10)
NKG2D ligand surface expression is important for immune recognition of stressed and neotransformed cells. In this study, we show that surface expression of MICA/B and other NKG2D ligands is dependent on N-linked glycosylation. The inhibitor of glycolysis and N-linked glycosylation
Mikael B L Winkler et al.
Cell, 179(2), 485-497 (2019-09-24)
Niemann-Pick type C (NPC) proteins are essential for sterol homeostasis, believed to drive sterol integration into the lysosomal membrane before redistribution to other cellular membranes. Here, using a combination of crystallography, cryo-electron microscopy, and biochemical and in vivo studies on the
Giel Detienne et al.
Experimental gerontology, 60, 129-135 (2014-12-03)
Royalactin is a glycoprotein essential for the development of long-lived queen honeybees. Only larvae fed with royal jelly, containing royalactin, develop into queens. Royalactin plays a central role in this process by switching on the epidermal growth factor (EGF) receptor
Yuzo Fujikura et al.
BMC biochemistry, 12, 52-52 (2011-09-23)
Hemojuvelin (HJV) is one of essential components for expression of hepcidin, a hormone which regulates iron transport. HJV is mainly expressed in muscle and liver, and processing of HJV in both tissues is similar. However, hepcidin is expressed in liver
문서
N-Linked Glycan Strategies; Sigma-Aldrich.com
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