Trypsin is a serine protease that is usually used in biochemistry and biology as an important enzymatic reagent. This method produces a highly purified trypsin product suitable for proteomics research. Proteomics Grade ideal for use in both solution and in-gel tryptic digestions. Trypsin, a serine protease, is present in the digestive system of several vertebrates.

Trypsin from porcine pancreas is used for the following applications:

• In-gel protein digestion and MALDI-TOF mass spectrometry analysis
• Electrospray Ionization Mass Spectrometry (ESI-MS) analysis
• Surface proteome profiling of L. plantarum
• Gel Filtration, Ultracentrifugation, and Rotary Shadowing Electron Microscopy
• Mass spectrometry

Trypsin is routinely used in proteomics research for peptide mapping and protein sequence work, due to its highly specific cleavage resulting in a limited number of tryptic peptides. It hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues.. The enzyme also exhibits esterase and amidase activities. Trypsin acts as a cell culture tool. It is used to hydrolyze allergenic proteins to produce hypoallergenic milk in industries.