MAK492

Fumarate Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC 코드: 12352200

속성

• Quality Level: 100
• 응용 분야: food and beverages
• 검출 방법: colorimetric
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Fumarate, or fumaric acid, is one of the key components in the TCA cycle and is used by cells to form ATP. Human skin, when exposed to sunlight, will naturally produce fumaric acid. Fumarate is used as an additive by the food and beverage industry. Fumaric acid esters are also used to treat psoriasis. Increased urinary fumarate may be due to impaired Krebs Cycle function, a defect in the enzyme fumarase, or mitochondrial function.

특징 및 장점

• Linear detection range 0.005 to 2 mM fumarate.
• Compatible with high-throughput handling systems.

적합성

The kit is suitable for the determination of fumarate in food, beverage, and other biological samples (e.g. cell lysate, tissue homogenate, and serum).

원리

The Fumarate Assay Kit is based on the fumarase catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH, which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm, is proportional to the fumarate concentration in the sample.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2