L5006

Leucine Aminopeptidase, microsomal from porcine kidney

Type IV-S, ammonium sulfate suspension, 10-40 units/mg protein (Bradford)

• 동의어(들): Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase
• CAS Number: 9054-63-1
• EC 번호: 3.4.11.2 (BRENDA, IUBMB)
• EC Number: 232-942-5
• MDL number: MFCD00131501
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Type IV-S
• Quality Level: 200
• 양식: ammonium sulfate suspension
• 특이 활성도: 10-40 units/mg protein (Bradford)
• 저장 온도: 2-8°C

설명

일반 설명

Leucine Aminopeptidase belongs to the family of metalloprotease.

애플리케이션

Leucine Aminopeptidase, microsomal from porcine kidney has been used in the isolation of neprilysin (NEP) from rodent brain-C5 and for peptide digestion.

생화학적/생리학적 작용

Leucine Aminopeptidase possesses broad substrate-specificity. It hydrolyzes peptides up to the proline residue and does not continue to degrade beyond proline.

단위 정의

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

물리적 형태

Suspension in 3.5 M (NH₄)₂SO₄ solution, pH 7.7, containing 10 mM MgCl₂

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)