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Merck
모든 사진(1)

주요 문서

I0138

Sigma-Aldrich

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in mouse

clone RG-16, ascites fluid

동의어(들):

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

secondary antibodies

클론

RG-16, monoclonal

포함

15 mM sodium azide

반응하면 안 됨

pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep

기술

indirect ELISA: 1:10,000

동형

IgG1

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

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관련 카테고리

일반 설명

Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins are the members of the eponymous immunoglobulin super-family (IgSF). Immunoglobulins are heterodimeric proteins characterized with two heavy (H) and two light (L) chains. Based on heavy chain C domains immunoglobulins are classified in to five main classes namely IgM, IgG, IgA, IgD, and IgE isotypes.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

특이성

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

애플리케이션

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA), immunoblots, dot blots and immunohistochemistry.
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

생화학적/생리학적 작용

Immunoglobulins acts as a cell-surface receptor for antigen that allows cell signaling and cell activation. In addition, it also acts as a soluble effector molecule that can recognize, bind and neutralize antigens. IgG contributes to immune response including neutralization of toxins and viruses. IgM provides first line of defense and is involved in immunoregulation. IgA protects mucous membranes against toxins, virus and bacteria by direct neutralization or by prevention of binding to the mucosal membranes.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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