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Merck
모든 사진(1)

주요 문서

H0137

Sigma-Aldrich

HT Media Supplement (50×) Hybri-Max

lyophilized powder, suitable for hybridoma, BioXtra

동의어(들):

HT Supplement, Hypoxanthine-Thymidin (HT) media supplement

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

제품명

HT Media Supplement (50×) Hybri-Max, lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma

Quality Level

무균

γ-irradiated

제품 라인

BioXtra

양식

lyophilized powder

기술

cell culture | hybridoma: suitable

불순물

endotoxin, tested

저장 온도

−20°C

애플리케이션

HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.

재구성

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.

법적 정보

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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Experimental Parasitology, 163, 46-56 (2016)
Simulated Cholinergic Reinnervation of beta (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing beta Cell and Cholinergic Cell
Jiao A, et al.
International Journal of Endocrinology, 2018 (2018)
The roles of human MTH1, MTH2 and MTH3 proteins in maintaining genome stability under oxidative stress
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Journal of bioscience and bioengineering, 128(4), 487-494 (2019-04-30)
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Protocol Guide: XTT Assay for Cell Viability and Proliferation

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