B5388

Brilliant Cresyl Blue ALD

Dye content, ≥60%, certified by the Biological Stain Commission, powder

• 동의어(들): 3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C, Brilliant Cresyl blue
• Linear Formula: C₁₇H₂₀N₃OCl · 1/2ZnCl₂
• CAS Number: 51716-96-2
• Molecular Weight: 385.96
• 색상 지수 번호: 51010
• Beilstein: 5690713
• EC Number: 279-675-0
• MDL number: MFCD00148901
• UNSPSC 코드: 12171500
• PubChem Substance ID: 24891836
• NACRES: NA.47

속성

• 제품명: Brilliant Cresyl Blue ALD, certified by the BSC
• Agency: certified by the BSC
• Quality Level: 200
• 양식: powder
• 구성: Dye content, ≥60%
• solubility: water: 1 mg/mL, blue to very deep blue
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: [Zn-2](Cl)(Cl)(Cl)Cl.N(CC)(CC)c4cc5[o+]c6c(nc5cc4)cc(c(c6)N)C.N(CC)(CC)c1cc2[o+]c3c(nc2cc1)cc(c(c3)N)C
• InChI: 1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4
• InChI key: SIHASHFMDFXGSX-UHFFFAOYSA-J

설명

애플리케이션

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).

생화학적/생리학적 작용

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

적합성

Certified as a supravital stain for reticulocytes.

기타 정보

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves