XTGHP-RO

X-tremeGENE^™ HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines

• UNSPSC 코드: 41106502
• NACRES: NA.55

속성

• Grade: Molecular Biology
• Quality Level: 100
• 양식: liquid (aqueous solution)
• 사용: 1 mL (suitable for 165 transfections)
• 포장: pkg of 0.4 mL (06366244001); pkg of 1.0 mL (06366236001); pkg of 5 × 1 mL (06366546001)
• 제조업체/상표: Roche
• 기술: transfection: suitable
• 저장 온도: −20°C

설명

일반 설명

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

특징 및 장점

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

• Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
• Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
• Generate physiologically relevant data using a reagent with low cytotoxic effects.
• Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

품질

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

물리적 형태

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

X-tremeGENE is a trademark of Roche

안전성 정보

• 픽토그램: GHS02,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H225 - H319
• 예방조치 성명서: P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point (°F): 334.4 °F
• Flash Point (°C): 168 °C