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Merck
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XTG9-RO

Roche

X-tremeGENE™ 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines

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모든 사진(6)

About This Item

UNSPSC 코드:
41106502
NACRES:
NA.55

Grade

Molecular Biology

Quality Level

100

양식

liquid (aqueous solution)

사용

1 mL (suitable for 165 transfections)

포장

pkg of 0.4 mL (06365779001)
pkg of 1.0 mL (06365787001)
pkg of 5 × 1.0 mL (06365809001)

제조업체/상표

Roche

기술

transfection: suitable

저장 온도

2-8°C

관련 카테고리

일반 설명

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

애플리케이션

X-tremeGENE™ 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE™ 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:
  • Expression of recombinant proteins for functional analysis.
  • Physiological studies of metabolic pathways.
  • Analysis of regulatory sequences using reporter gene assays.
  • Gene expression assays.
  • Cancer research studies.
  • Target evaluation.

특징 및 장점

  • Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
  • Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
  • Avoid time-consuming optimization procedures in commonly used cell lines.

품질

Each lot of X-tremeGENE™ 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE™ 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

물리적 형태

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

기타 정보

For life science research only. Not for use in diagnostic procedures.

법적 정보

X-tremeGENE is a trademark of Roche

관련 제품

제품 번호
설명
가격

D5796

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

R8758

RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

NA0300

GenElute™ HP Plasmid Maxiprep Kit, sufficient for 10 purifications

NA0400

GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit, sufficient for 10 preparations

PP2393

Mammalian FLAG® Tag Vector Set, plasmid vectors for molecular cloning

XTGHP-RO

X-tremeGENE™ HP DNA Transfection Reagent, High-performance polymer reagent for transfecting many cell lines

SITRAN-RO

X-tremeGENE™ siRNA Transfection Reagent, Polymer reagent for delivering siRNA to common cell lines

픽토그램

FlameExclamation mark
GHS02,GHS07

신호어

Danger

유해 및 위험 성명서

H225 - H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

334.4 °F

Flash Point (°C)

168 °C

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
19129300
39320900
46261800
33315000
23644720

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COA 검색

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Sigma-Aldrich

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모든 관련된 서류 보기

문서

Reverse transfection of plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical gene knockout techniques.

Introduction to Cell Transfection

Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions, protein synthesis, cell growth and development.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

프로토콜

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

관련 콘텐츠

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

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