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Merck
모든 사진(1)

주요 문서

모든 문서 보기

11718096001

Roche

DAB Substrate

dark brown, /black precipitate; Visually evaluated

동의어(들):

3,3′-diaminobenzidine tetrahydrochloride, DAB

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모든 사진(1)

About This Item

UNSPSC 코드:
12352204

제조업체/상표

Roche

Quality Level

100

색상

dark brown, /black precipitate; Visually evaluated

저장 온도

−20°C

관련 카테고리

일반 설명

The metal enhanced DAB Substrate utilizes cobalt chloride and nickel chloride in a special formulation to produce a dark brown/black precipitate in the presence of horseradish peroxidase (HRP).
3, 3′ diaminobenzidine tetrahydrochloride (DAB) is a carcinogen that should be used safely. It is one of the major sensitive substrates for HRP (horseradish peroxidase). DAB is brown or amber in color and becomes insoluble and precipitates over the antigen-antibody site due to the presence of hydrogen peroxide and peroxidase.

애플리케이션

  • DAB Substrate is a color sustrate for peroxidases and is used in a variety of applications:Immunohistocytochemistry
  • In situ hybridization
  • Western blot
  • Dot blot
DAB substrate has been used in immunofluorescence and western blot.

포장

1 set containing 2 components.

제조 메모

Working solution:
  1. Determine the volume of substrate required for the development of peroxidase.
  2. Remove the DAB/metal concentrate (10x) from -15 to -25 °C storage.
    Note: Do not bring to 15 to 25 °C.
  3. Dilute the DAB/metal concentrate (10x) with the peroxide buffer to a 1x working solution. For example, if 5 ml of substrate are required, dilute 500 μl of the DAB/metal concentrate (10x) with 4.5 ml of the Peroxide buffer.
  4. Mix well.
  5. Add the 1x working solution to the tissue or blot until desired substrate development.
    Note: The 1x solution is stable over many hours.
    For best results, store the working solution at 2 to 8 °C when not using.

Storage conditions (working solution): 2 to 8 °C
For best results, store the working solution at 2 to 8 °C when not using.

기타 정보

For life science research only. Not for use in diagnostic procedures.

키트 구성품 전용

제품 번호
설명
  • DAB/metal concentrate, 25 ml 10x concentrated
  • Peroxide Buffer, 250 ml

신호어

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 - Aquatic Chronic 2 - Carc. 1A - Carc. 1B - Flam. Liq. 2 Oral - Repr. 1B - Skin Sens. 1 Inhalation - STOT RE 2 - STOT SE 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

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시험 성적서(COA)

Lot/Batch Number
70300900
64173700
73360000
67308700
60286000

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