추천 제품
양식
solution
Quality Level
사용
sufficient for 20 reactions
포장
pkg of 40 μL
제조업체/상표
Roche
기술
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
저장 온도
−20°C
일반 설명
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
특이성
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
애플리케이션
Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
- in situ hybridization to tissues
- Southern blots
- northern blots
- plaque or colony lifts
- RNase protection experiments
품질
The mix is function tested in combination with the DIG RNA Labeling Kit.
원리
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
제조 메모
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
No data available
Flash Point (°C)
No data available
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문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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