추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
clone 1.71.256, monoclonal
양식
lyophilized
포장
pkg of 100 μg
제조업체/상표
Roche
기술
ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL
동형
IgG1κ
저장 온도
2-8°C
일반 설명
Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.
특이성
The monoclonal antibody reacts with free and bound digoxigenin.
애플리케이션
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
- Immunofluorescence staining
- FISH (fluorescent in situ hybridization)
생화학적/생리학적 작용
In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.
특징 및 장점
- Specific to free and bound digoxigenin
- The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.
제조 메모
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: For coating applications
phosphate buffered saline, pH 7.4
- ELISA: for coating: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications
phosphate buffered saline, pH 7.4
재구성
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
저장 및 안정성
The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.
분석 메모
There are no cross reactivities known.
기타 정보
For life science research only. Not for use in diagnostic procedures.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
이미 열람한 고객
Nonradioactive labeling of probe with digoxigenin by polymerase chain reaction
<BIG>Lion T and Haas OA</BIG>
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Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+-ATPase by N-Hydroxysuccinimidyl Derivatives of Digoxigenin
<BIG>Antolovic R, et al.</BIG>
European Journal of Biochemistry, 227, 61-67 (1995)
Xingqi Chen et al.
BioTechniques, 56(3), 117-118 (2014-03-20)
Current techniques for analyzing chromatin structures are hampered by either poor resolution at the individual cell level or the need for a large number of cells to obtain higher resolution. This is a major problem as it hampers our understanding
문서
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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