추천 제품
생물학적 소스
mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)
Quality Level
무균
sterile; sterile-filtered
분석
≥90% (SDS-PAGE)
양식
solution
분자량
900 kDa
포장
pkg of 1 mg (2 ml)
제조업체/상표
Roche
기술
cell culture | mammalian: suitable
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
mouse ... Lamb2(16779)
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일반 설명
Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.
Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.
Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.
애플리케이션
Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
품질
Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)
서열
Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.
물리적 형태
Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane
제조 메모
Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
이미 열람한 고객
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H Colognato et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 218(2), 213-234 (2000-06-08)
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문서
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
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