추천 제품
제품명
Anti-Chondroitin 4 Sulfate Antibody, clone BE-123, ascites fluid, clone BE-123, Chemicon®
생물학적 소스
mouse
Quality Level
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
BE-123, monoclonal
종 반응성
human, canine, rat, sheep, bovine, mouse
제조업체/상표
Chemicon®
기술
ELISA: suitable
radioimmunoassay: suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... CSPG4(1464)
특이성
Reacts with both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin-4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase.
면역원
Mouse proteoglycan.
애플리케이션
Anti-Chondroitin 4 Sulfate Antibody, clone BE-123 detects level of Chondroitin 4 Sulfate & has been published & validated for use in ELISA, RIA & WB.
ELISA at 1:400-1:3,200.
RIA at 1:50-1:6,400.
Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)
Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.
Optimal working dilutions must be determined by the end user.
RIA at 1:50-1:6,400.
Immunohistochemistry: This antibody only reacts with digested material. Fixed tissue must be digested with chondroitinase ABC (Sigma).
The Reference 23757342 should be indicated for IHC. (Reine, TM., et al., PubMed ID: 23757342)
Western Blot: 1:1000-1:4000 using ECL detection method. Chondroitinase ABC treatment of lysate sample is helpful.
In any alkali buffer, e.g. Tris or acetate (50mM or 100mM) pH 7.4-7.6. Make a stock solution by diluting one vial of Sigma Chondroitinase A, B, C, (5 units/vial) in 100 µL . For use, dilute stock 10-fold into the buffer of choice. Use at a ratio of 50-100mg of proteoglycan per 5 units of enzyme. On fixed cells, dilute in Tris acetate and on non-fixed cellsy dilute 10-25 fold in DMEM.
Poole, C.A., et al., J. Histochem. Cytochem. 1991. 39:1175-1187.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
물리적 형태
Ascites fluid containing no preservatives.
저장 및 안정성
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A human schwannoma cell line supports the in vitro adhesion of Plasmodium falciparum infected erythrocytes to chondroitin-4-sulfate.
K T Andrews, N K Viebig, F Wissing, N Klatt, N Oster, H Wickert, P Knolle, M Lanzer
Parasitology Research null
Cytoadherence of Plasmodium falciparum-infected erythrocytes in the human placenta.
B Maubert, N Fievet, G Tami, C Boudin, P Deloron
Parasite Immunology null
Effect of cytokine treatment on the in vitro expression of the P. falciparum adhesion receptor chondroitin-4-sulphate on the surface of human choriocarcinoma (BeWo) cells.
Yvonne Adams,Reinhard Schwartz-Albiez,James S McCarthy,Katherine T Andrews
Parasitology Research null
Efficient secretion of biologically active Chondroitinase ABC from mammalian cells in the absence of an N-terminal signal peptide.
Michael Kluppel
Molecular and Cellular Biochemistry null
Adherence of Plasmodium falciparum infected erythrocytes to CHO-745 cells and inhibition of binding by protein A in the presence of human serum.
K T Andrews, Y Adams, N K Viebig, M Lanzer, R Schwartz-Albiez
International Journal For Parasitology null
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