ABE251

Anti-N-CoR Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Nuclear receptor corepressor 1, N-CoR, N-CoR1, Retinoid X receptor-interacting protein 13
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human
• 기술: western blot: suitable
• NCBI 수납 번호: NP_035438
• UniProt 수납 번호: Q60974
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NCOR1(9611)

설명

일반 설명

In the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes.

면역원

KLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.

애플리케이션

Anti-N-CoR Antibody is a Rabbit Polyclonal Antibody for detection of N-CoR also known as Nuclear receptor corepressor 1 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

품질

Evaluated by Western Blot in RAW 264.7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected N-CoR in RAW 264.7 cell lysate. No tween for best results.

표적 설명

~270 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at MW of 266 kDa and 270 kDa

결합

Replaces: 06-892

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
RAW 264.7 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable