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Merck
모든 사진(1)

주요 문서

AB5374

Sigma-Aldrich

Anti-Potassium Channel Kir2.1 Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

rat

제조업체/상표

Chemicon®

기술

immunohistochemistry: suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... KCNJ2(3759)

특이성

Recognizes Kir2.1 protein (IRK1, Kcnj2). The epitope is specific for Kir2.1 and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is identical in rabbit, bovine, porcine and guinea pig. It is highly conserved in rat and mouse (17/19 residues), chick (15/19 residues), and pigeon (14/18). Other species have not been tested.

면역원

Purified peptide from human Kir2.1 (amino acids 392-410) (Accession number P48049).

애플리케이션

Detect Potassium Channel Kir2.1 using this Anti-Potassium Channel Kir2.1 Antibody validated for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot: 1:200 using ECL on rat brain and rat heart membranes. Note: Addition of 0.1% Tween to the standard milk block is recommended for this lot.

Immunohistochemistry on rat heart sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

물리적 형태

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

저장 및 안정성

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

분석 메모

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


시험 성적서(COA)

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문서 라이브러리 방문

Fadi G Akar et al.
American journal of physiology. Heart and circulatory physiology, 288(6), H2887-H2896 (2005-02-01)
Heart failure (HF) is characterized by marked prolongation of action potential duration and reduction in cellular repolarization reserve. These changes are caused in large part by HF-induced K(+) current downregulation. Molecular mechanisms underlying these changes remain unclear. We determined whether
Michael Kristensen et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 290(3), R766-R772 (2005-10-15)
Muscle activity is associated with potassium displacements, which may cause fatigue. It was reported previously that the density of the large-conductance Ca2+-dependent K+ (BK(Ca)) channel is higher in the T tubule membrane than in the sarcolemmal membrane and that the
Yunping Deng et al.
The Journal of comparative neurology, 529(7), 1327-1371 (2020-09-02)
We used behavioral testing and morphological methods to detail the progression of basal ganglia neuron type-specific pathology and the deficits stemming from them in male heterozygous Q175 mice, compared to age-matched WT males. A rotarod deficit was not present in
Thomas P Gunnarsson et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 305(7), R811-R821 (2013-07-26)
The effects of intensified training in combination with a reduced training volume on muscle ion kinetics, transporters, and work capacity were examined. Eight well-trained cyclists replaced their regular training with speed-endurance training (12 × 30 s sprints) 2-3 times per

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