251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• 동의어(들): DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• 실험식(Hill 표기법): C₂₅H₁₈F₂N₂O₇
• CAS Number: 254109-22-3
• Molecular Weight: 496.42
• MDL number: MFCD16872050
• UNSPSC 코드: 12352204
• NACRES: NA.25

속성

• Quality Level: 100
• 분석: ≥98% (HPLC)
• 양식: solution
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: pale yellow
• 형광: λ_ex ~500 nm; λ_em ~515 nm
• 저장 온도: −20°C
• SMILES string: Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• InChI key: BEVHTVRRVVEMEF-UHFFFAOYSA-N

설명

일반 설명

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

생화학적/생리학적 작용

Primary Target
Nitric oxide (NO) fluorescent indicator

경고

Toxicity: Irritant (B)

물리적 형태

A 5 mM solution in DMSO.

재구성

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

기타 정보

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

법적 정보

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flash Point (°C): 87 °C - closed cup - (Dimethylsulfoxide)