219373

Cathepsin G, Human Neutrophil

Cathepsin G, Human Neutrophil, CAS 107200-92-0, is a purified native cathepsin G. Acts as a potent agonist of human platelet activation leading to their aggregation.

• 동의어(들): Cathepsin G, Human Neutrophil
• CAS Number: 107200-92-0
• EC 번호: 3.4.21.20 (BRENDA, IUBMB)
• MDL number: MFCD00130750
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: human neutrophils
• Quality Level: 100
• 설명: Merck USA index - 14, 1905
• 분석: ≥95% (SDS-PAGE)
• 양식: lyophilized solid (Salt-free)
• 특이 활성도: ≥2 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 기술: inhibition assay: suitable
• 적합성: suitable for molecular biology
• 응용 분야: life science and biopharma
• 배송 상태: ambient
• 저장 온도: −20°C
• 유전자 정보: human ... CTSG(1511)

설명

일반 설명

Research area: Cell Signaling

Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.

애플리케이션

Cathepsin G, Human Neutrophil has been used to determine the in vitro bactericidal activities of cathepsin G and for in vitro digestion of plasma/acute wound fluids.

생화학적/생리학적 작용

This protease cleaves the triple-helix structure of type I collagen in a restricted manner and degrades nonhelical regions of type I and II collagens, fibronectin, aggrecan, and elastin. Additionally, cathepsin G activates ProMMP-3 and -8. Cathepsin G serves as a chemoattractant for human phagocytes and enhances the random motility of T cells. Additionally, it initiates chemotaxis, activates ERK1/2 and p38 MAPK, and facilitates the translocation of PKCζ to the cell membrane, without inducing a detectable calcium flux. Other biological functions of cathepsin G include: ECM components and plasma proteins degradation, bactericidal actions, cleavage of inflammatory mediators, conversion of angiotensin I to angiotensin II, platelet activation and induction of airway submucosal gland secretion.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-AAPF-pNA per min at 25°C, pH 7.5.

제조 메모

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.

재구성

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -70°C.

Reconstitute in 50 mM NaOAc and 150 mM NaCl, pH 5.5.

기타 정보

Glusa, E., and Adam C. 2001. Por. J. Pharmacol.133, 422.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable