07-1533
Anti-JMJD3 (NT) Antibody
from rabbit
동의어(들):
JMJD, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, jumonji domain containing 3, jumonji domain containing 3, histone lysine demethylase
로그인조직 및 계약 가격 보기
모든 사진(3)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... KDM6B(23135)
일반 설명
JMJD3 (Jumonji domain-containing protein 3) is part of the family of novel JmjC domain-containing histone demethylation (JHDM) enzymes. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. JMJD3 specifically demethylates Lys27 on histone H3, demethylating both the trimethylated and dimethylated form. JMJD3 plays a role in the regulation of posterior development by regulating HOX gene expression. It is also involved in the inflammatory response by regulating gene expression and macrophage differentiation
특이성
Not tested in other species.
Recognizes the N-terminal region of human JMJD3. Additional lower MW bands are detected in some lysates that may be degradation products.
면역원
Epitope: N-terminus
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JMJD3.
애플리케이션
ELISA:
This antibody is reported by an
outside laboratory to be suitable for ELISA.
Immunohistochemistry:
This antibody is reported by an outside laboratory to be suitable for immuno-histochemistry.
Immunocytochemistry Analysis:
Confocal immunofluorescence analysis of HeLa and NIH/3T3 using 1:100 dilution of anti-JMJD3 (N-Terminus) (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green).
Western Blot Analysis:
The antibody recognizes JMJD3 in HeLa cell lysates.
This antibody is reported by an
outside laboratory to be suitable for ELISA.
Immunohistochemistry:
This antibody is reported by an outside laboratory to be suitable for immuno-histochemistry.
Immunocytochemistry Analysis:
Confocal immunofluorescence analysis of HeLa and NIH/3T3 using 1:100 dilution of anti-JMJD3 (N-Terminus) (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green).
Western Blot Analysis:
The antibody recognizes JMJD3 in HeLa cell lysates.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Chromatin Biology
Histone Modifying Proteins
Chromatin Biology
Use Anti-JMJD3 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ICC, ELISA, WB to detect JMJD3 (N-terminus) also known as JmjC domain-containing protein 3.
품질
Immunocytochemisty:
Recognizes JMJD3 in HeLa, NIH 3T3, and A431 cells by cytochemistry (1:50 – 1:100 dilution).
Recognizes JMJD3 in HeLa, NIH 3T3, and A431 cells by cytochemistry (1:50 – 1:100 dilution).
표적 설명
~180 kDa
물리적 형태
Format: Purified
Protein G Chromatography
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
HeLa cells
HeLa cells
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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