G1N10

GenElute^™ Mammalian Genomic DNA Miniprep Kits

sufficient for 10 purifications

• Synonyma: Mammalian Genomic DNA Miniprep, Gen Elute
• UNSPSC Code: 41105501
• NACRES: NA.55

Vlastnosti

• usage: sufficient for 10 purifications
• Quality Level: 200
• storage temp.: 15-25°C

Popis

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• ligation

Features and Benefits

• Expected yield: 25 μg from 2 x 10⁶ cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• Mechanical homogenization required: No

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Součásti soupravy, které jsou dostupné také samostatně

• C2112 Column Preparation Solution
• P2308 Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein
• R6148 RNase A solution

Bezpečnostní informace

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H290,H302,H315,H318,H334,H335,H410
• pcodes: P234 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Skladovací třída: 8A - Combustible corrosive hazardous materials
• flash_point_f: 483.8 °F
• flash_point_c: 251 °C