SAE0045
HRV-3C Protease.
N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL
Synonyme(s) :
Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
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About This Item
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Source biologique
human (human Rhinovirus Type 14)
Niveau de qualité
Essai
≥90% (SDS-PAGE)
Forme
aqueous solution
Activité spécifique
≥5000 U/mg
Poids mol.
21 kDa
Concentration
0.8-1.2 mg/mL
Technique(s)
protein purification: suitable
Adéquation
suitable for protein modification
Application(s)
life science and biopharma
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.
Application
HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.
Définition de l'unité
One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.
Forme physique
Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
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