SAB5900004

Alpaca Anti-HA single domain antibody, magnetic beads

recombinant Alpaca(VHH) Nano antibody magnetic bead suspension

Propriétés

• Source biologique: alpaca
• Niveau de qualité: 100
• Produit recombinant: expressed in Pichia pastoris
• Conjugué: magnetic beads conjugate
• Type de produit anticorps: primary antibodies
• Clone: recombinant monoclonal
• Forme: liquid
• Technique(s): ChIP: suitable; RIP: suitable; co-immunoprecipitation (co-IP): suitable; immunoprecipitation (IP): suitable; mass spectrometry (MS): suitable
• Couleur: black
• Matrice: (~45 um (cross-linked to 7.5% magarose beads))
• Isotype: VHH
• Capacité: binding capacity (10 μL slurry bind about 2ug-3μg of HA tagged protein)
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

HA-tag (YPYDVPDYA) has been extensively used as a general epitope tag, which does not generally interfere with the bioactivity or the biodistribution of the recombinant protein. This tag greatly facilitates the detection, isolation and purification of the protein of interest.Anti-HA single domain antibody, magnetic beads are magnetic agarose spheres covalently coupled with VHH antibodies acquiring high specificity and affinity for HA tag. These beads can efficiently capture and separate HA tagged proteins, alongside with the associated proteins from cell extracts of mammal, plant, bacteria, yeast, insect, and other organisms.

Spécificité

Selectively recognizes HA fusion protein

Immunogène

HA Tagged Protein

Caractéristiques et avantages

• Anti Tag-Nano antibody beads are single-domain/variable region of heavy chain of camelid antibodies(sdAb/VHH).
• They are directly coupled to beads covalently.
• They have unique properties such as small size (~15kDa, 2-4nm) when compared to conventional antibodies.
• No heavy and light chains in downstream interactions.
• Acids and alkali resistance, tolerance to harsh conditions.
• Short incubation time 5 – 30 minutes.
• Low batch to batch variations.
• Typically, antibodies are not detectable in Western Blot.

Conditionnement

plastic tube

Forme physique

1XPBS, 0.03% sodium azide, 50% glycerol.

Autres remarques

Before usage, please invert the vial several times (DO NOT VORTEX) to form beads suspension, and take out beads’ suspension using a pipette tip with bigger opening (i.e., cut the tip of a 1000 μl pipette tip with sterile scissors). Once opened, it is recommended to use paraffin film to seal the cap.