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SAB4200660

Sigma-Aldrich

Monoclonal Anti-GOLPH3 antibody produced in mouse

clone GOL3-1, purified from hybridoma cell culture

Synonyme(s) :

GOPP1, GPP34, MIDAS, golgi phosphoprotein 3 (coat-protein)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

GOL3-1

Forme

buffered aqueous solution

Poids mol.

antigen ~34 kDa

Espèces réactives

human, mouse, rat

Concentration

~1 mg/mL

Technique(s)

immunoblotting: 2.5-5 μg/mL using using whole extracts of rat PC-12 cells.
immunofluorescence: 2.5-5 μg/mL using mouse 3T3 cells
immunoprecipitation (IP): 5-10 μg using using lysates of human HeLa cells

Isotype

IgG1

Numéro d'accès UniProt

Q9H4A6

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... GOLPH3(64083)
mouse ... Golph3(66629)
rat ... Golph3(78961)

Description générale

GOLPH3 (golgi phosphoprotein 3) is an abundant phosphatidylinositol-4-phosphate (PtdIns4P)-interacting protein which is conserved from yeast to humans. This protein is localized to Golgi through PtdIns4P. This gene is localized to human chromosome 5p13. GOLPH3 has a molecular weight of 34kDa.
Monoclonal Anti-GOLPH3 (mouse IgG1 isotype) is derived from the hybridoma GOL3-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. GOLPH3 (Golgi phosphoprotein 3) is a peripheral membrane protein of the Golgi stack that localizes to the trans-Golgi network. GOLPH3 is conserved from yeast to human. This gene was described as an oncogene that is commonly amplified in human cancer and in cancer cell lines.

Immunogène

synthetic peptide corresponding to a sequence at the N-terminal region of human Golph3

Application

Monoclonal Anti-GOLPH3 antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Actions biochimiques/physiologiques

GOLPH3 (golgi phosphoprotein 3) links Golgi membrane to actin cytoskeleton by interacting with MYO18A, which is an unconventional myosin. This, in turn, is crucial for Golgi architecture and vesicle trafficking. It is an oncogene, which is susceptible to amplification in cancers, and is responsible for controlling the response to the anti-cancer drug, rapamycin. It is thought to function as a coat protein during Golgi trafficking.
GOLPH3 has a role in Golgi trafficking and morphology. It interacts with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. GOLPH3 is also required for Golgi function. Overexpression of GOLPH3 is correlated with hyperactivation of mTOR signaling, in human cancer cells.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
035M4840V

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Consulter la Bibliothèque de documents

Yingchun Ma et al.
Virchows Archiv : an international journal of pathology, 464(4), 443-452 (2014-01-25)
Overexpression of GOLPH3 (Golgi phosphoprotein 3, 34 kDa) is associated with the progression of many solid tumor types leading to an unfavorable clinical outcome. The present study examined the association between GOLPH3 expression and tumor development, clinicopathological factors, and prognosis
GOLPH3 links the Golgi network to mTOR signaling and human cancer.
Robert T Abraham
Pigment cell & melanoma research, 22(4), 378-379 (2009-07-25)
A W Bell et al.
The Journal of biological chemistry, 276(7), 5152-5165 (2000-10-24)
A mass spectrometric analysis of proteins partitioning into Triton X-114 from purified hepatic Golgi apparatus (84% purity by morphometry, 122-fold enrichment over the homogenate for the Golgi marker galactosyl transferase) led to the unambiguous identification of 81 proteins including a
Kenneth L Scott et al.
Nature, 459(7250), 1085-1090 (2009-06-26)
Genome-wide copy number analyses of human cancers identified a frequent 5p13 amplification in several solid tumour types, including lung (56%), ovarian (38%), breast (32%), prostate (37%) and melanoma (32%). Here, using integrative analysis of a genomic profile of the region
Holly C Dippold et al.
Cell, 139(2), 337-351 (2009-10-20)
Golgi membranes, from yeast to humans, are uniquely enriched in phosphatidylinositol-4-phosphate (PtdIns(4)P), although the role of this lipid remains poorly understood. Using a proteomic lipid-binding screen, we identify the Golgi protein GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p)

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