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M8787

Sigma-Aldrich

Chlorure de magnésium solution

PCR Reagent, 25 mM MgCI2 solution for PCR

Synonyme(s) :

PCR optimization

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About This Item

Formule linéaire :
MgCl2
Numéro CAS:
7786-30-3
Poids moléculaire :
95.21
Numéro MDL:
MFCD00011106
Code UNSPSC :
12352302
ID de substance PubChem :
57654422
Nomenclature NACRES :
NA.52

Niveau de qualité

200
300

Forme

liquid

Conditionnement

vial of 1.5 mL

Concentration

25 mM±1 mM

Technique(s)

PCR: suitable

Couleur

colorless

Application(s)

agriculture
diagnostic assay manufacturing

Activité étrangère

DNase, RNase, none detected

Température de stockage

2-8°C

Chaîne SMILES 

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

Clé InChI

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Catégories apparentées

Description générale

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Application

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression

Adéquation

Suitable for optimization of polymerase chain reactions.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCK6176
SLCH7551
SLCC7461
SLBZ9143
SLBQ4119V

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Kadri Õunap et al.
PloS one, 10(7), e0133841-e0133841 (2015-07-28)
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the protein function in ribosome synthesis is independent of its enzymatic activity. In this work, we have
Manal A Farg et al.
Human molecular genetics, 23(13), 3579-3595 (2014-02-20)
Intronic expansion of a hexanucleotide GGGGCC repeat in the chromosome 9 open reading frame 72 (C9ORF72) gene is the major cause of familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. However, the cellular function of the C9ORF72 protein remains unknown.
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
Takeshi Tomita et al.
PloS one, 9(9), e108957-e108957 (2014-10-01)
Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins
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