L6533
Lectin from Dolichos biflorus (horse gram)
biotin conjugate, buffered aqueous solution
Synonyme(s) :
Dolichos biflorus agglutinin, DBA
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Conjugué
biotin conjugate
Niveau de qualité
Forme
buffered aqueous solution
Composition
Protein, ≥2.5 mg/mL biuret
Ampleur du marquage
≥3 mol biotin per mol lectin
Température de stockage
−20°C
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Catégories apparentées
Description générale
Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.
Application
Lectin from Dolichos biflorus (horse gram) has been used:
- to characterize mouse uterine natural killer (uNK) cells
- in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
- as a species-specific gonocyte marker for the determination of donor cell colonization
- to label undifferentiated spermatogonia/cat testicular tissue
Actions biochimiques/physiologiques
DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.
Conditionnement
Sold on the basis of mg active conjugate.
Forme physique
contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells
Nakano H, et al.
Reproduction (Cambridge, England), 124(4), 581-592 (2002)
Rajesh Dash et al.
JACC. Cardiovascular interventions, 11(2), 195-205 (2018-01-20)
The study investigated whether a dose response exists between myocardial salvage and the depth of therapeutic hypothermia. Cardiac protection from mild hypothermia during acute myocardial infarction (AMI) has yielded equivocal clinical trial results. Rapid, deeper hypothermia may improve myocardial salvage.
Analysis of uterine natural killer cells in mice
Croy BA, et al.
Natural Killer Cell Protocols, 465-503 (2010)
B Anne Croy et al.
Methods in molecular biology (Clifton, N.J.), 612, 465-503 (2009-12-25)
The term uterine natural killer (uNK) cell is applied in mice to an abundant but transient NK cell population that undergoes unique, terminal differentiation within embryo implantation sites during endometrial decidualization and pregnancy. In mice, decidualization is induced by attachment
Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
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