G5003

α-Glucosidase from Saccharomyces cerevisiae

Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

• Synonyme(s) : α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast
• Numéro CAS: 9001-42-7
• Numéro CE : 3.2.1.20 (BRENDA, IUBMB)
• Numéro CE : 232-604-7
• Numéro MDL: MFCD00081321
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type I
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
• Contient: phosphate buffer salts and EDTA as balance
• Composition: Protein, ≥50%
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Numéro d'accès UniProt: P38158; P53341
• Activité étrangère: β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%
• Autre catégorie plus écologique: , Enabling
• Maladie(s) pertinente(s): diabetes (management of non-insulin-dependent diabetes mellitus)
• Température de stockage: −20°C
• Informations sur le gène: bakers yeast ... MAL12(853209) , MAL32(852602)

Description

Description générale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Application

α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.

For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters

Actions biochimiques/physiologiques

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.

Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.

Conditionnement

Sold on basis of p-nitrophenyl α-D-glucoside units.

Définition de l'unité

1 U setzt 1.0 μmol D-Glucose aus p-Nitrophenyl α-D-glucosid pro Minute frei bei pH 6.8 und 37°C.

Remarque sur l'analyse

Protein determined by biuret.

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)