F1554

Anti-FXR2 antibody, Mouse monoclonal

clone A42, purified from hybridoma cell culture

• Numéro MDL: MFCD02262924
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: A42, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~74 kDa
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 2-4 μg/mL using total cell extract of HEK 293T cells
• Isotype: IgG1
• Numéro d'accès UniProt: P51116
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FXR2(9513)

Description

Description générale

Monoclonal Anti-FXR2 (mouse IgG1 isotype) is derived from the hybridoma A42 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with human Fragile X mental retardation syndrome-related protein 2 (FXR2) recombinant protein. FXR2 gene is located on human chromosome 17 and its protein is localized mainly in the cytoplasm. FXR2 is expressed along with other proteins in the cytoplasm of neurons. It is characterized with two KH domains and one RGG box that together with fragile X mental retardation 1 (FMR1) bind to RNA. FXR2 is highly expressed in brain and testis.

Spécificité

Monoclonal Anti-FXR2 recognizes human, monkey, bovine, canine, rat, hamster, and mouse, FXR2, ~74 kDa, and does not crossreact with FMR or FXR1.

Immunogène

human FXR2 recombinant protein.

Application

Anti-FXR2 antibody, Mouse monoclonal may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• co-immunoprecipitation
• immunofluorescence
• sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

Actions biochimiques/physiologiques

Fragile X mental retardation syndrome-related protein 2 (FXR2) is capable of forming heteromers with the others and can also form homomers. It has an independent function in tissues during embryonic development and adult life.

Fragile X mental retardation syndrome-related protein 2 (FXR2) knockout mice are hyperactive in the open-field test, impaired on the rotarod test, have reduced levels of prepulse inhibition, display less contextual conditioned fear, are impaired at locating the hidden platform in the Morris water task, and less sensitive to heat stimulus.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2–8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable