Accéder au contenu
MilliporeSigma
Toutes les photos(2)

Principaux documents

Voir toute la documentation

E8159

Sigma-Aldrich

Anti-EDEM1 antibody,Mouse monoclonal

clone EDEM1-17, purified from hybridoma cell culture

Synonyme(s) :

Anti-EDEM, Anti-ER degradation enhancer, mannosidase alpha-like 1

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

EDEM1-17, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen 75/65 kDa

Espèces réactives

mouse, human

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells expressing recombinant human or mouse EDEM1

Isotype

IgG2a

Numéro d'accès UniProt

Q92611

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... EDEM1(9695)

Description générale

Monoclonal Anti-EDEM1 (mouse IgG2a isotype) is derived from the hybridoma EDEM1-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human EDEM1. conjugated to keyhole limpet hemocyanin (KLH). EDEM1 (ER degradation-enhancing alphamannosidase-like protein 1) is a putative mannose binding lectin. In mammalian cells, EDEM1 is localized to the endoplasmic reticulum (ER), mainly as a soluble glycoprotein.

Application

Monoclonal Anti-EDEM1 antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • immuno-electron microscopy
  • immunohistochemistry (IHC)

Actions biochimiques/physiologiques

EDEM1 (ER degradation-enhancing alphamannosidase-like protein 1) has the ability to interact with the COOH-terminus of calnexin. It lacks mannosidase activity.
ER degradation-enhancing α-mannosidase-like 1 is an enzyme encoded by the EDEM1 gene in humans. It is a chaperone for rod opsin and its expression can be used to promote correct folding enhancing degradation of mutant proteins in the ER to combat protein-misfolding disease. The endogenous EDEM1 in cells that are not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy. EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation. It is also considered responsible for directing aberrant proteins for ERAD (ER-associated protein degradation).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
094M4800
089K4812

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Yu-Qun Wang et al.
Carcinogenesis, 35(9), 2127-2133 (2014-06-11)
Hepatitis B virus surface antigen (HBsAg) is an important risk factor for hepatocellular carcinoma (HCC) and is downregulated during hepatocarcinogenesis. MicroRNAs (miRNAs) are frequently deregulated in HCC tissues. However, whether the deregulation of certain miRNAs in HCC has an impact
Nobuko Hosokawa et al.
Glycobiology, 20(5), 567-575 (2010-01-13)
Glycoprotein folding and degradation in the endoplasmic reticulum (ER) is mediated by the ER quality control system. Mannose trimming plays an important role by forming specific N-glycans that permit the recognition and sorting of terminally misfolded conformers for ERAD (ER-associated
Christine Insinna et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(23), 8094-8104 (2012-06-08)
In absence of their natural ligand, 11-cis-retinal, cone opsin G-protein-coupled receptors fail to traffic normally, a condition associated with photoreceptor degeneration and blindness. We created a mouse with a point mutation (F81Y) in cone S-opsin. As expected, cones with this
EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites
Zuber C, et al.
Proceedings of the National Academy of Sciences of the USA, 104(11), 4407-4412 (2007)
Human EDEM2, a novel homolog of family 47 glycosidases, is involved in ER-associated degradation of glycoproteins
Mast SW, et al.
Glycobiology, 15(4), 421-436 (2004)
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique