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D9310

Sigma-Aldrich

Glycerol-free JumpStart™ Taq DNA Polymerase

Lyophilization-compatible

Synonyme(s) :

Lyo-ready JumpStart™ Taq, Lyo-ready hot-start Taq

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.55

Source biologique

mouse ascites (recombinant)

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Forme

liquid

Caractéristiques

hotstart

Concentration

5 unit/μL

Technique(s)

PCR: suitable

Densité

1 g/cm3

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Glycerol-Free JumpStart™ Taq DNA Polymerase is a lyophilization-compatible blend of Sigma′s high-performance Taq DNA Polymerase and JumpStart™ Taq antibody. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70 °C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set up with less contamination risk than manual hot-start techniques. The enzyme may also be included in the master mix preparation resulting in more consistency from one reaction to the next.
Glycerol-free JumpStart™ Taq DNA Polymerase is suitable for lyophilization and allows the user to lyophilize PCR master mixes, which can be stored at ambient temperature and used up to one year later without reagent degradation.

Application

Typical applications for Glycerol-Free JumpStart™ Taq DNA polymerase include lyophilization of PCR master mixes and any other DNA amplification protocol in which glycerol is contraindicated, such as freeze-drying and automated PCR applications.

Caractéristiques et avantages

  • Designed to minimize non-specific amplification
  • Increased specificity and target yield
  • Reduced primer-dimers
  • Reduced set-up time as compared to manual or wax Hot Start methods
  • Allows room-temperature reaction set-up
  • Suitable for multiplex PCR
  • Lyophilization-compatible hot-start enzyme that is glycerol-free

Conditionnement

The enzyme is supplied at 5 units/μL in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.05 mM EDTA, 5 mM DTT, and stabilizers.

Définition de l'unité

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Autres remarques

View more detailed information on JumpStart Taq enzymes at our Hot Start PCR page.

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Lot/Batch Number

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