CMC0014
BL21(DE3) Chemically Competent Cells
Escherichia coli, rod shaped
Synonyme(s) :
Competent E. coli cells
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About This Item
Code UNSPSC :
41106202
Nomenclature NACRES :
NA.85
Produits recommandés
Nom du produit
BL21(DE3) Chemically Competent Cells, for protein expression
Source biologique
Escherichia coli
Qualité
Molecular Biology
Mode de croissance
adherent or suspension
Morphologie
rod shaped
Technique(s)
microbiological culture: suitable
Transformation cellulaire
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
Conditions d'expédition
dry ice
Température de stockage
−70°C
Description générale
The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Application
The E. coli strain BL21 (DE3) has been used:
- to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
- BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
- to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.
Caractéristiques et avantages
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
Composants
- BL21(DE3) chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
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