TPS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96112839, fish kidney (anterior), Fibroblast-like/Epithelial-like

• Code UNSPSC : 41106514

Propriétés

• Source biologique: fish kidney (anterior)
• Conditionnement: tube of 5 μg 96112839-DNA-5UG; pkg of vial of cells 96112839-1VL
• Mode de croissance: Adherent
• Caryotype: Modal no. 58
• Morphologie: Fibroblast-like/Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Fish Rainbow Trout Pronephric Stroma

Description de la lignée cellulaire

TPS has been initiated from a long-term pronephric stroma culture of an adult 1 year old rainbow trout (Oncorhynchus mykiss W). The cells grow at 4° to 22°C, with an optimum temperature of 18°C. They do not form true monolayers, but overlap in a multilayered network of fibroblastic, epithelioid and giant cells. TPS cells are susceptible to viral haemorrhagic septicemia virus (VHSV) and infectious haemopoietic necrosis virus (IHNV). They have been applied to support the development of haemopoiesis when co-cultured with haemopoietic precursors. Spontaneous angiogenesis processes in vitro occurred in TPS cultures when they are kept in the same flask, i.e. without passages, for long times (over 2 months).

Application

Fish Haemopoiesis and Fish pathology.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 25mM Hepes + 2.5mM NaP + 0.01mM Hydrocortisone-21-hemisuccinate + 0.088mM Guanosine + 0.0225mM Cytidine + 0.094mM Adenosine + 0.102mM Uridine + 10% FBS + 10% HS, pH 7.4-7.6. At ECACC successful growth of this cell line has bee

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; trypsinise at room temperature once a week; air atmosphere; 18°C. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.