Hs 27

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human prostate
• Conditionnement: tube of 5 μg 94041901-DNA-5UG; pkg of vial of cells 94041901-1VL
• Mode de croissance: Adherent
• Caryotype: 46XY
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human foreskin, fibroblast

Description de la lignée cellulaire

Hs27 is one of a series of human foreskin fibroblast lines developed at NBL, California. The material was obtained from a normal new-born, and the cells possess the G6PD type A phenotype. Longevity studies carried out at NBL demonstrated that the cells can be propagated for 42 passages. There were no consistent aberrations in analysed cells. Ethnicity: Black.

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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